5g5t

Structure of the Argonaute protein from Methanocaldcoccus janaschii in complex with guide DNAStructure of the Argonaute protein from Methanocaldcoccus janaschii in complex with guide DNA

Structural highlights 5g5t is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: , Related: 5g5s Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Argonaute (Ago) proteins in eukaryotes are known as key players in post-transcriptional gene silencing1, while recent studies on prokaryotic Agos hint at their role in the protection against invading DNA2,3. Here, we present crystal structures of the apo enzyme and a binary Ago-guide complex of the archaeal Methanocaldococcus jannaschii (Mj) Ago. Binding of a guide DNA leads to large structural rearrangements. This includes the structural transformation of a hinge region containing a switch helix, which has been shown for human Ago2 to be critical for the dynamic target search process4-6. To identify key residues crucial for MjAgo function, we analysed the effect of several MjAgo mutants. We observe that the nature of the 3' and 5' nucleotides in particular, as well as the switch helix, appear to impact MjAgo cleavage activity. In summary, we provide insights into the molecular mechanisms that drive DNA-guided DNA silencing by an archaeal Ago. Structural and mechanistic insights into an archaeal DNA-guided Argonaute protein.,Willkomm S, Oellig CA, Zander A, Restle T, Keegan R, Grohmann D, Schneider S Nat Microbiol. 2017 Mar 20;2:17035. doi: 10.1038/nmicrobiol.2017.35. PMID:28319084 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Argonaute

spinqualitylabelsall models 5g5t, resolution 2.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image