3tz1

Crystal structure of the Ca2+-saturated C-terminal domain of Akazara scallop troponin C in complex with a troponin I fragmentCrystal structure of the Ca2+-saturated C-terminal domain of Akazara scallop troponin C in complex with a troponin I fragment

Structural highlights 3tz1 is a 2 chain structure with sequence from Akazara scallop. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Gene: Troponin C (Akazara scallop) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [TNNI_CHLNI] Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Publication Abstract from PubMed Abstract Troponin C (TnC) is the Ca2+-sensing subunit of troponin that triggers the contraction of striated muscles. In scallops, the striated muscles consume little ATP energy in sustaining strong contractile forces. The N-terminal domain of TnC works as the Ca2+ sensor in vertebrates, whereas scallop TnC uses the C-terminal domain as the Ca2+ sensor, suggesting that there are differences in the mechanism of the Ca2+-dependent regulation of muscles between invertebrates and vertebrates. Here, we report the crystal structure of the Akazara scallop adductor muscle TnC C-terminal domain (asTnCC) complexed with a short troponin I fragment (asTnIS) and Ca2+. The electron density of a Ca2+ ion is observed in only one of the two EF-hands. The EF-hands of asTnCC can only be in the fully open conformation with the assistance of asTnIS. The number of hydrogen bonds between asTnCC and asTnIS is markedly lower than the number in the vertebrate counterparts. The Ca2+ modulation on the binding between asTnCC and asTnIS is weaker, but structural change of the complex depending on Ca2+ concentration was observed. Together, these findings provide a detailed description of the distinct molecular mechanism of contractile regulation in the scallop adductor muscle from that of vertebrates. Structure of the Ca2+-saturated C-terminal domain of scallop troponin C in complex with a troponin I fragment.,Kato YS, Yumoto F, Tanaka H, Miyakawa T, Miyauchi Y, Takeshita D, Sawano Y, Ojima T, Ohtsuki I, Tanokura M Biol Chem. 2012 Aug 4. pii:, /j/bchm.just-accepted/hsz-2012-0152/hsz-2012-0152.xml. doi:, 10.1515/hsz-2012-0152. PMID:23096565 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Troponin

spinqualitylabelsall models 3tz1, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image