Samatey/3

Interactive 3D Complement in Proteopedia

Scientific Reports an online, open access journal: nature.com/srep

Structural insights into bacterial flagellar hook similarities and specifities.
Young-Ho Yoon, Clive S. Barker, Paula V. Bulieris, Hideyuki Matsunami, and Fadel A. Samatey.
Scientific Reports in press 6:35552, 2016: nature.com/articles/srep35552. (DOI: 10.1038/srep35552)

The interactive Molecular Tour below assumes that you are familiar with the journal article.

Molecular TourMolecular Tour

Monomer Structures Campylobacter jejuni The structure of the flagellar hook monomer FlgE2 (gene Cj1729c) of Campylobacter jejuni () was determined to resolution 2.45 Å (5az4). FlgE2 of C. jejuni (FlgE2-Cj; Q0P7Q2_CAMJE) consists of 865 amino acids. The fragment crystallized was residues 91-828 (738 residues, 85% of full length). The crystallographic model has coordinates for residues 94-819 (726 residues, 84% of full length). The 79 kD fragment crystallized will be referred to as FlgE2-Cj79. N               C N->C Rainbow Color Scheme FlgE2-Cj is composed of (D0-D4). D0 was not present in the fragment FlgE2-Cj79 in order to facilitate crystallization. D1-3 consist of discontinuous segments of the single protein chain, while D4 is one continuous segment. Here is a scene . The domains . D3 contains three alpha-helices while D4 contains one. Alpha Helices,  Beta Strands  Caulobacter crescentus The structure of the flagellar hook monomer FlgE (gene flgE) of Caulobacter cresentus Å (5ay6). N               C N->C Rainbow Color Scheme FlgE of C. crecentus (FlgE-Cc; P35806, FLGE_CAUCR, gene CC_0902) consists of 591 amino acids. The fragment crystallized was residues 165-470 (306 residues, 52% of full length). The crystallographic model has coordinates for residues 167-469 (303 residues, 51% of full length). The fragment crystallized is 32 kD, and will be referred to as FlgE-Cc32. FlgE-Cc32 forms domains D2 and D3. D0 and D1 were removed to facilitate crystallization. D2 is formed by two discontinuous segments, while D3 is a single continuous segment inserted between the segments of D2. Here the model is . Comparison with Salmonella FlgE (gene STM1177) of Salmonella enterica serotype Typhimurium (FlgE-St, P0A1J1_SALTY) contains 403 amino acids, compared to the 865 in FlgE-Cj. However, the D1 and D2 domains are very similar in the two species (FlgE-St42: 1wlg). This animation takes ~2 minutes. Reload the page to cancel. FlgE-Cj contains the additional domains D3 and D4, making it a "hook with a hat". A small adjustment in the angle between D1 and D2 in FlgE-Cj79 (a reduction of about 12o) enables with FlgE-St42 (RMSD for 137 of 287 alpha carbons: 0.94 Å). The adjustment can also be . There is also close structural similarity between domains D1 and D2 comparing FlgE-Cc32 with FlgE-St42, and comparing Flg-Cj79 with Flg-Cc32 (Supplementary Fig. 3 in the journal article).

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