1e6e
ADRENODOXIN REDUCTASE/ADRENODOXIN COMPLEX OF MITOCHONDRIAL P450 SYSTEMS
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OverviewOverview
The steroid hydroxylating system of adrenal cortex mitochondria consists, of the membrane-attached NADPH-dependent adrenodoxin reductase (AR), the, soluble one-electron transport protein adrenodoxin (Adx), and a, membrane-integrated cytochrome P450 of the CYP11 family. In the 2.3-A, resolution crystal structure of the Adx.AR complex, 580 A(2) of partly, polar surface are buried. Main interaction sites are centered around, Asp(79), Asp(76), Asp(72), and Asp(39) of Adx and around Arg(211), Arg(240), Arg(244), and Lys(27) of AR, respectively. In particular, the, region around Asp(39) defines a new protein interaction site for Adx, similar to those found in plant and bacterial ferredoxins. Additional, contacts involve the electron transfer region between the redox centers of, AR and Adx and C-terminal residues of Adx. The Adx residues Asp(113) to, Arg(115) adopt 3(10)-helical conformation and engage in loose, intermolecular contacts within a deep cleft of AR. Complex formation is, accompanied by a slight domain rearrangement in AR. The [2Fe-2S] cluster, of Adx and the isoalloxazine rings of FAD of AR are 10 A apart suggesting, a possible electron transfer route between these redox centers. The AR.Adx, complex represents the first structure of a biologically relevant complex, between a ferredoxin and its reductase.
About this StructureAbout this Structure
1E6E is a Protein complex structure of sequences from Bos taurus with SO4, FAD and FES as ligands. The following page contains interesting information on the relation of 1E6E with [Cytochrome p450]. Active as Ferredoxin--NADP(+) reductase, with EC number 1.18.1.2 Structure known Active Sites: AC1, AC5, AC6 and AC9. Full crystallographic information is available from OCA.
ReferenceReference
Adrenodoxin reductase-adrenodoxin complex structure suggests electron transfer path in steroid biosynthesis., Muller JJ, Lapko A, Bourenkov G, Ruckpaul K, Heinemann U, J Biol Chem. 2001 Jan 26;276(4):2786-9. Epub 2000 Oct 25. PMID:11053423 [[Category: [2fe-2s]ferredoxin]]
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