3wg2

Crystal structure of Agrocybe cylindracea galectin mutant (N46A)Crystal structure of Agrocybe cylindracea galectin mutant (N46A)

Structural highlights 3wg2 is a 2 chain structure with sequence from Agrae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Related: 3wg1, 3wg3, 3wg4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed A fungal galectin from Agrocybe cylindracea (ACG) exhibits broad binding specificity for beta-galactose-containing glycans. We determined the crystal structures of wild-type ACG and the N46A mutant, with and without glycan ligands. From these structures and a saccharide-binding analysis of the N46A mutant, we revealed that a conformational change of a unique insertion sequence containing Asn46 provides two binding modes for ACG, and thereby confers broad binding specificity. We propose that the unique sequence provides these two distinct glycan-binding modes by an induced-fit mechanism. Conformational change of a unique sequence in a fungal galectin from Agrocybe cylindracea controls glycan ligand-binding specificity.,Kuwabara N, Hu D, Tateno H, Makyio H, Hirabayashi J, Kato R FEBS Lett. 2013 Nov 15;587(22):3620-5. doi: 10.1016/j.febslet.2013.08.046. Epub, 2013 Sep 10. PMID:24036446 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 3wg2, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image