3vuf

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Crystal Structure of Rice Granule bound Starch Synthase I Catalytic Domain in Complex with ADPCrystal Structure of Rice Granule bound Starch Synthase I Catalytic Domain in Complex with ADP

Structural highlights

3vuf is a 1 chain structure with sequence from Japanese rice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:,
Gene:WAXY, WX, WX-B, Os06g0133000, LOC_Os06g04200, 134P10.7, P0679C08.19 (Japanese rice)
Activity:NDP-glucose--starch glucosyltransferase, with EC number 2.4.1.242
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SSG1_ORYSJ] Required for the synthesis of amylose in endosperm.[HAMAP-Rule:MF_00484]

Publication Abstract from PubMed

The catalytic domain of rice (Oryza sativa japonica) granule bound starch synthase I (OsGBSSI-CD) was overexpressed and the three-dimensional structures of the ligand-free and ADP-bound forms were determined. The structures were similar to those reported for bacterial and archaeal glycogen synthases, which belong to glycosyltransferase family 5. They had Rossmann fold N- and C-domains connected by canonical two-hinge peptides, and an interdomain disulfide bond that appears to be conserved in the Poaceae plant family. The presence of three covalent linkages might explain why both OsGBSSI-CD structures adopted only the closed domain arrangement.

Interdomain Disulfide Bridge in the Rice Granule Bound Starch Synthase I Catalytic Domain as Elucidated by X-Ray Structure Analysis.,Momma M, Fujimoto Z Biosci Biotechnol Biochem. 2012 Aug 7. PMID:22878205[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Momma M, Fujimoto Z. Interdomain Disulfide Bridge in the Rice Granule Bound Starch Synthase I Catalytic Domain as Elucidated by X-Ray Structure Analysis. Biosci Biotechnol Biochem. 2012 Aug 7. PMID:22878205

3vuf, resolution 3.00Å

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