4ftx

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Crystal structure of Ego3 homodimerCrystal structure of Ego3 homodimer

Structural highlights

4ftx is a 2 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:SLM4, EGO3, GSE1, YBR077C, YBR0723 (Baker's yeast)
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SLM4_YEAST] Component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Component of the EGO complex, a complex involved in the regulation of microautophagy.[1] [2]

Publication Abstract from PubMed

The yeast EGO complex, consisting of Gtr1, Gtr2, Ego1, and Ego3, localizes to the endosomal and vacuolar membranes and plays a pivotal role in cell growth and autophagy regulation through relaying amino acid signals to activate TORC1. Here, we report the crystal structures of a wild-type and a mutant form of Saccharomyces cerevisiae Ego3. Ego3 assumes a homodimeric structure similar to that of the mammalian MP1-p14 heterodimer and the C-terminal domains of the yeast Gtr1-Gtr2 heterodimer, both of which function in TORC1 signaling. Structural and genetic data demonstrate that the unique dimer conformation of Ego3 is essential for the integrity and function of the EGO complex. Structural and functional data also identify a potential binding site for Gtr1-Gtr2. These results suggest a structural conservation of the protein components involved in amino acid signaling to TORC1 and reveal structural insights into the molecular mechanism of Ego3 function in TORC1 signaling.

Ego3 Functions as a Homodimer to Mediate the Interaction between Gtr1-Gtr2 and Ego1 in the EGO Complex to Activate TORC1.,Zhang T, Peli-Gulli MP, Yang H, De Virgilio C, Ding J Structure. 2012 Oct 30. pii: S0969-2126(12)00372-3. doi:, 10.1016/j.str.2012.09.019. PMID:23123112[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Dubouloz F, Deloche O, Wanke V, Cameroni E, De Virgilio C. The TOR and EGO protein complexes orchestrate microautophagy in yeast. Mol Cell. 2005 Jul 1;19(1):15-26. PMID:15989961 doi:10.1016/j.molcel.2005.05.020
  2. ↑ Gao M, Kaiser CA. A conserved GTPase-containing complex is required for intracellular sorting of the general amino-acid permease in yeast. Nat Cell Biol. 2006 Jul;8(7):657-67. Epub 2006 May 28. PMID:16732272 doi:ncb1419
  3. ↑ Zhang T, Peli-Gulli MP, Yang H, De Virgilio C, Ding J. Ego3 Functions as a Homodimer to Mediate the Interaction between Gtr1-Gtr2 and Ego1 in the EGO Complex to Activate TORC1. Structure. 2012 Oct 30. pii: S0969-2126(12)00372-3. doi:, 10.1016/j.str.2012.09.019. PMID:23123112 doi:10.1016/j.str.2012.09.019

4ftx, resolution 2.10Å

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