2cky
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Structure of the arabidopsis thaliana thiamine pyrophosphate riboswitch with its regulatory ligand
OverviewOverview
Riboswitches are untranslated regions of messenger RNA, which adopt, alternate structures depending on the binding of specific metabolites., Such conformational switching regulates the expression of proteins, involved in the biosynthesis of riboswitch substrates. Here, we present, the 2.9 angstrom-resolution crystal structure of the eukaryotic, Arabidopsis thaliana thiamine pyrophosphate (TPP)-specific riboswitch in, complex with its natural ligand. The riboswitch specifically recognizes, the TPP via conserved residues located within two highly distorted, parallel "sensor" helices. The structure provides the basis for, understanding the reorganization of the riboswitch fold upon TPP binding, and explains the mechanism of resistance to the antibiotic pyrithiamine.
About this StructureAbout this Structure
2CKY is a Single protein structure of sequence from [1] with MG, OS and TPP as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the eukaryotic thiamine pyrophosphate riboswitch with its regulatory ligand., Thore S, Leibundgut M, Ban N, Science. 2006 May 26;312(5777):1208-11. Epub 2006 May 4. PMID:16675665
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