2j7n
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STRUCTURE OF THE RNAI POLYMERASE FROM NEUROSPORA CRASSA
OverviewOverview
RNA silencing refers to a group of RNA-induced gene-silencing mechanisms, that developed early in the eukaryotic lineage, probably for defence, against pathogens and regulation of gene expression. In plants, protozoa, fungi, and nematodes, but apparently not insects and vertebrates, it, involves a cell-encoded RNA-dependent RNA polymerase (cRdRP) that produces, double-stranded RNA triggers from aberrant single-stranded RNA. We report, the 2.3-A resolution crystal structure of QDE-1, a cRdRP from Neurospora, crassa, and find that it forms a relatively compact dimeric molecule, each, subunit of which comprises several domains with, at its core, a catalytic, apparatus and protein fold strikingly similar to the catalytic core of the, DNA-dependent RNA polymerases responsible for transcription. This, evolutionary link between the two enzyme types suggests that aspects of, RNA silencing in some organisms may recapitulate transcription/replication, pathways functioning in the ancient RNA-based world.
About this StructureAbout this Structure
2J7N is a Single protein structure of sequence from Neurospora crassa with MG and GOL as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
ReferenceReference
The structure of an RNAi polymerase links RNA silencing and transcription., Salgado PS, Koivunen MR, Makeyev EV, Bamford DH, Stuart DI, Grimes JM, PLoS Biol. 2006 Dec;4(12):e434. PMID:17147473
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