Barnase

Function Barnase (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium Bacillus amyloliquefaciens. The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA. Relevance BA structure was used extensively for studying protein folding. Structural highlights BA hydrolyzes RNA at GpN sites where the active site positively charged Lys and Arg coordinate the RNA phosphate group.

spinqualitylabelsall models Barnase complex with tetranucleotide (PDB code 1brn) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of Barnase3D Structures of Barnase

Updated on 09-November-2015

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• Barnase
  • 3kch, 2f4y – BA cross-linked
    
  • 2f56 - BA cross-linked + urea
    
  • 2f5w - BA cross-linked + thiourea
    
  • 2f5m - BA cross-linked + Bromo-ethanol
    
  • 1fw7, 1bnr – BA - NMR
    
  • 2kf3 – BA (mutant) – NMR
    
  • 2kf4, 1b2x, 1a2p, 1bni, 1bnj – BA
    
  • 1yvs – BA domain swapped
    
  • 1b20, 1b21, 1b2z, 1bne, 1bnf, 1bng, 1brh, 1bri, 1brj, 1brk, 1bns, 1brg, 1bsa, 1bsb, 1bsc, 1bsd, 1bse, 1ban, 1bao – BA (mutant)
    
  • 2kf5, 2kf6, 1brn, 1rnb – BA + polynucleotide
• Barnase + BST
  • 3da7 - BA (mutant) + BST
    
  • 2za4, 1brs, 1bgs – BA + BST
    
  • 1x1u, 1x1w, 1x1x, 1x1y, 1b27, 1b2u – BA + BST (mutant)
    
  • 1b3s, 1b2s - BA (mutant) + BST (mutant)
    
  • 2c4b – BA/MCOEETI (mutant)