2fa1
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| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | phnF (Escherichia coli) | ||||||
| Domains: | UTRA | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of PhnF C-terminal domain
OverviewOverview
The crystal structure of Escherichia coli PhnF C-terminal domain (C-PhnF) was solved at 1.7 A resolution by the single wavelength anomalous dispersion (SAD) method. The PhnF protein belongs to the HutC subfamily of the large GntR transcriptional regulator family. Members of this family share similar N-terminal DNA-binding domains, but are divided into four subfamilies according to their heterogenic C-terminal domains, which are involved in effector binding and oligomerization. The C-PhnF structure provides for the first time the scaffold of this domain for the HutC subfamily, which covers about 31% of GntR-like regulators. The structure represents a mixture of alpha-helices and beta-strands, with a six-stranded antiparallel beta-sheet at the core. C-PhnF monomers form a dimer by establishing interdomain eight-strand beta-sheets that include core antiparallel and N-terminal two-strand parallel beta-sheets from each monomer. C-PhnF shares strong structural similarity with the chorismate lyase fold, which features a buried active site locked behind two helix-turn-helix loops. The structural comparison of the C-PhnF and UbiC proteins allows us to propose that a similar site in the PhnF structure is adapted for effector binding.
About this StructureAbout this Structure
2FA1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Structural characterization of GntR/HutC family signaling domain., Gorelik M, Lunin VV, Skarina T, Savchenko A, Protein Sci. 2006 Jun;15(6):1506-11. Epub 2006 May 2. PMID:16672238
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OCA- Pages with broken file links
- Escherichia coli
- Single protein
- Edwards, A M.
- Gorelik, M.
- Lunin, V V.
- MCSG, Midwest Center for Structural Genomics.
- Nocek, B P.
- Savchenko, A.
- Skarina, T.
- Apc5558
- Effector binding domain
- Mcsg
- Midwest center for structural genomic
- Pnhf
- Protein structure initiative
- Psi
- Regulator
- Transcription