1l3a
Structure of the plant transcriptional regulator PBF-2Structure of the plant transcriptional regulator PBF-2
Structural highlights
Function[WHY1_SOLTU] Single-stranded DNA-binding protein that acts as a transcriptional activator of the pathogenesis-related gene PR-10a. Upon elicitation, binds a 30bp promoter sequence known as elicitor element response (ERE) and is required for PR-10a expression.[1] [2] [3] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe crystal structure of p24, the single-stranded DNA (ssDNA) binding subunit of the plant defense transcription factor PBF-2, has been determined to 2.3 A resolution. p24 is representative of a novel family of ubiquitous plant-specific proteins that we refer to as the Whirly family because of their quaternary structure. PBF-2 is composed of four p24 molecules that interact through a helix-loop-helix motif. This interaction produces a central pore, with beta-strands radiating outwards, resulting in a whirligig appearance to the quaternary structure. The noncrystallographic C(4) symmetry arrangement of p24 subunits is novel for ssDNA binding proteins and may explain the binding specificity of PBF-2. This structural arrangement also supports the role of PBF-2 in binding melted promoter regions to modulate gene expression. A new family of plant transcription factors displays a novel ssDNA-binding surface.,Desveaux D, Allard J, Brisson N, Sygusch J Nat Struct Biol. 2002 Jul;9(7):512-7. PMID:12080340[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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