1y7h
| |||||||
| , resolution 2.52Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Domains: | MhpC | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural and biochemical studies identify tobacco SABP2 as a methylsalicylate esterase and further implicate it in plant innate immunity, Northeast Structural Genomics Target AR2241
OverviewOverview
Salicylic acid (SA) is a critical signal for the activation of plant defense responses against pathogen infections. We recently identified SA-binding protein 2 (SABP2) from tobacco as a protein that displays high affinity for SA and plays a crucial role in the activation of systemic acquired resistance to plant pathogens. Here we report the crystal structures of SABP2, alone and in complex with SA at up to 2.1-A resolution. The structures confirm that SABP2 is a member of the alpha/beta hydrolase superfamily of enzymes, with Ser-81, His-238, and Asp-210 as the catalytic triad. SA is bound in the active site and is completely shielded from the solvent, consistent with the high affinity of this compound for SABP2. Our biochemical studies reveal that SABP2 has strong esterase activity with methyl salicylate as the substrate, and that SA is a potent product inhibitor of this catalysis. Modeling of SABP2 with MeSA in the active site is consistent with all these biochemical observations. Our results suggest that SABP2 may be required to convert MeSA to SA as part of the signal transduction pathways that activate systemic acquired resistance and perhaps local defense responses as well.
About this StructureAbout this Structure
1Y7H is a Single protein structure of sequence from Nicotiana tabacum. Full crystallographic information is available from OCA.
ReferenceReference
Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity., Forouhar F, Yang Y, Kumar D, Chen Y, Fridman E, Park SW, Chiang Y, Acton TB, Montelione GT, Pichersky E, Klessig DF, Tong L, Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1773-8. Epub 2005 Jan 24. PMID:15668381
Page seeded by OCA on Wed Mar 26 06:13:53 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Nicotiana tabacum
- Single protein
- Acton, T B.
- Chen, Y.
- Chiang, Y.
- Forouhar, F.
- Fridman, E.
- Klessig, D F.
- Kumar, D.
- Montelione, G T.
- NESG, Northeast Structural Genomics Consortium.
- Park, S W.
- Pichersky, E.
- Tong, L.
- Yang, Y.
- Alpha-beta protein
- Nesg
- Northeast structural genomics consortium
- Protein structure initiative
- Psi
- Structural genomic