1mjh
| |||||||
| , resolution 1.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | MJ0577 (Methanocaldococcus jannaschii) | ||||||
| Domains: | USP_Like | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE-BASED ASSIGNMENT OF THE BIOCHEMICAL FUNCTION OF HYPOTHETICAL PROTEIN MJ0577: A TEST CASE OF STRUCTURAL GENOMICS
OverviewOverview
Many small bacterial, archaebacterial, and eukaryotic genomes have been sequenced, and the larger eukaryotic genomes are predicted to be completely sequenced within the next decade. In all genomes sequenced to date, a large portion of these organisms' predicted protein coding regions encode polypeptides of unknown biochemical, biophysical, and/or cellular functions. Three-dimensional structures of these proteins may suggest biochemical or biophysical functions. Here we report the crystal structure of one such protein, MJ0577, from a hyperthermophile, Methanococcus jannaschii, at 1.7-A resolution. The structure contains a bound ATP, suggesting MJ0577 is an ATPase or an ATP-mediated molecular switch, which we confirm by biochemical experiments. Furthermore, the structure reveals different ATP binding motifs that are shared among many homologous hypothetical proteins in this family. This result indicates that structure-based assignment of molecular function is a viable approach for the large-scale biochemical assignment of proteins and for discovering new motifs, a basic premise of structural genomics.
About this StructureAbout this Structure
1MJH is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
ReferenceReference
Structure-based assignment of the biochemical function of a hypothetical protein: a test case of structural genomics., Zarembinski TI, Hung LW, Mueller-Dieckmann HJ, Kim KK, Yokota H, Kim R, Kim SH, Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15189-93. PMID:9860944
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- Methanocaldococcus jannaschii
- Single protein
- BSGC, Berkeley Structural Genomics Center.
- Hung, L W.
- Kim, K K.
- Kim, R.
- Kim, S H.
- Mueller-Dieckmann, H J.
- Yokota, H.
- Zarembinski, T I.
- Atp binding protein
- Berkeley structural genomics center
- Bsgc structure funded by nih
- Functional assignment
- Hypothetical protein
- Protein structure initiative
- Psi
- Structural genomic