Ryanodine receptor

Ryanodine receptors (RyR) are the largest (over 5000 residues) ion channels and are responsible for the release of Ca+2 ions from the sarco/endoplasmic reticulum. RyR is a homotetramer which opens upon binding of Ca+2. RyR is modulated by various ions, small molecules and proteins. The protein modulators include calmodulin, FKBP, protein phosphatase and protin kinase A. The C-terminal of RyR forms the transmembrane ion-conducting pore. The N-terminal of RyR named “foot”, includes a trefoil knot fold and interacts with the various modulators. The corners of the RYR cytoplasmic area are called “clamp” and undergo conformational change upon opening or closing of the channel. RYR domains include three SPRY domains. SPRY2 forms the link between the N terminal gating ring and the clamp region. RYR1 and RYR2 have several phosphorylation sites. Ryanodine is a plant alkaloid which binds to RyR with high affinity and locks the channel in an open state. There are 3 RyR isoforms. RyR1 is found in skeletal muscles, RyR2 is found in cardiac muscles and RyR3 is found in the brain. Disease Mutations in RyR are associated with skeletal muscle disorders and triggered cardiac arrhythmia. The N-terminal of RYR was found to be the disease hot spot of the molecule.

spinqualitylabelsall models Rabbit ryanodine receptor 1 N-terminal (PDB code 2xoa) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of ryanodine receptor3D Structures of ryanodine receptor

Updated on 13-July-2015

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• Ryanodine receptor 1
  • 3hsm, 3ila – rRyR1 N terminal b-trefoil domain residues 1-210 – rabbit
    
  • 2xoa – rRyR1 N terminal residues 1-559
    
  • 4i0y, 4i1e, 4i2s, 4i37, 4i3n, 4i6i, 4i8m – rRyR1 N terminal residues 1-559 (mutant)
    
  • 4i96 – rRyR1 N terminal residues 217-536
    
  • 4p9j – rRyR1 SPRY2 domain residues 1070-1246
    
  • 3rqr – rRyR1 phosphorylation domain residues 2733-2940 + tetrapeptide
    
  • 4ert – rRyR1 phosphorylation domain residues 2733-2940
    
  • 4esu, 4ett, 4etu – rRyR1 phosphorylation domain residues 2733-2940 (mutant)
    
  • 4uwa, 4uwe – rRyR1 – Cryo EM
    
  • 3j8e – rRyR1 + FKBP1B – Cryo EM
    
  • 3j8h – rRyR1 + FKBP12 – EM
    
• Ryanodine receptor 2 (Cardiac Ca+2 release channel)
  • 3im5 – mRyR2 N terminal residues 1-217 – mouse
    
  • 3im6, 3im7, 4kei, 4kej, 4kek – mRyR2 N terminal residues 1-217 (mutant)
    
  • 2mc2 – mRyR2 N terminal residues 1-217 - NMR
    
  • 3qr5 – mRyR2 N terminal residues 1-56, 92-217
    
  • 4etv – mRyR2 phosphorylation domain residues 2699-2904 (mutant)
    
  • 4l4h – mRyR2 N terminal residues 1-547
    
  • 4l4i – mRyR2 N terminal residues 1-547 (mutant)
    
  • 4p9i – mRyR2 SPRY2 domain residues 1080-1253
    
  • 4p9l – mRyR2 SPRY2 domain residues 1080-1253 (mutant)
    
  • 4jkq – hRyR2 N terminal residues 1-606 - human
    
• Ryanodine receptor 3
  • 4erv – hRyR3 residues 2597-2800