Proteinase

Proteinase (PRO) are enzymes which hydrolyze peptide bonds. They are classified by the amino acid site of their cleavage or by the pH at which they are active. PRO B is a serine protease. For more details see Streptomyces griseus proteinase B. PRO A is a carboxylproteinase. PRO K is a serine protease which cleaves proteins preferentially after hydrophobic residues. Calcium ions contribute to the stability of the enzyme. PRO K is active over a wide pH range and is used in molecular biology to inactivate nucleases from preparations of DNA or RNA. PRO K is used in the partial proteolysis of lactoferrin into its N- and C-lobe. The two lobes of lactoferrin have different antimicrobial and antifungal properties. PRO K can digest hair (keratin).

spinqualitylabelsall models E. coli proteinase K (grey) complex with lactoferrin peptide (green) and Ca+2 (green) and nitrate ions (PDB code 2duj) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D structures of proteinase3D structures of proteinase

Updated on 21-May-2015

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• Proteinase A
  • 2sga – SgPRO – Streptomyces griseus
    
  • 2jxr, 1fmu, 1fmx – yPRO - yeast
    
  • 1sgc - SgPRO + chymostatin A
    
  • 3sga, 4sga, 5sga - SgPRO + polypeptide inhibitor
    
  • 1dp5, 1dpj, 1g0v - yPRO + polypeptide inhibitor IA3
    
  • 1fq5 - yPRO + inhibitor
    
  • 4fvd – hevPRO 2A + peptide – human enterovirus
    
  • 4fvb – hevPRO 2A (mutant)
    
• Proteinase B
  • 3sgb – SgPRO + turkey ovomucoid inhibitor
    
  • 1sgp, 1sgq, 1sgr, 1cso, 1ct0, 1ct2, 1ct4, 1ds2, 2sgp, 2nu3, 2nu4 – SgPRO + turkey ovomucoid inhibitor (mutant)
    
  • 4sgb - SgPRO + potato inhibitor
• Proteinase K
  • 2prk, 1cnm, 1egq, 2id8, 2g4v, 2v8b, 3gt3, 3gt4, 3d9q, 3ddz, 3de0 , 3de1, 3de2, 3de3, 3de4, 3de5, 3de6, 3de7, 3dvq, 3dvr, 3dvs, 3dw1, 3dw3, 3dwe, 3i2y, 3i30, 3i37, 3i34, 3l1k, 3aj8, 3aj9, 3q40, 3q5g, 3qmp, 4b5l, 4fon – EaPRO + Ca – Engyodontium album
    
  • 1ic6 – EaPRO (mutant) + Ca
    
  • 1ptk, 1ht3 – EaPRO + Ca + Hg
    
  • 2pkc – EaPRO + Na
    
  • 4dj5, 4woc, 4wob – EaPRO
    
• Proteinase K complex with peptide
  • 3prk, 1p7v, 1p7w – EaPRO + Ca + peptide inhibitor
    
  • 1bjr, 2dqk, 2duj – EaPRO + Ca + lactoferrin peptide
    
  • 2hd4 – EaPRO + Ca + lactoferrin peptide inhibitor
    
  • 2dp4, 3ptl – EaPRO + lactoferrin peptide
    
  • 1pek, 1pfg, 4zar – EaPRO + peptide inhibitor
    
  • 1pj8 – EaPRO + Hg + substrate analog peptide
    
  • 2hpz, 2pq2 – EaPRO + Ca + peptide
    
  • 3osz – EaPRO + Ca + antimicrobial peptide
    
  • 2b6n – PRO + tripeptide - Serratia
    
• Proteinase K complex with small molecule
  • 2pwb – EaPRO + Ca + coumarin
    
  • 2pyz – EaPRO + Ca + auramine
    
  • 2pwa – EaPRO + Ca + alanine boronic acid
    
  • 1oyo – EaPRO + Ca + melanin monomer
    
  • 3dyb – EaPRO + Ca + digalacturonic acid
    
• Proteinase 3C
  • 1qa7 – PRO – Hepatitis virus
    
  • 2vb0, 3zyd - PRO – Coxsakievirus
    
  • 3zye – csvPRO (mutant)
    
  • 3zzd, 3zzc, 3zza, 3zzb, 3zz8, 3zz9, 3zz7, 3zz5, 3zz3, 3zz4 – csvPRO (mutant) + inhibitor
    
  • 4ghq – hevPRO
    
  • 4ght – hevPRO + common cold drug
    
• H2-Proteinase
  • 1wni – PRO – Trimeresurus flavoviridis
• Aspartic Proteinase
  • 2asi – PRO – Rhizomucor miehei
    
  • 1zap – CaPRO – Candida albicans
    
  • 1izd - AoPRO – Aspergillus oryzae
    
  • 1eag – CaPRO + inhibitor
    
  • 1fq4 - yPRO + inhibitor
    
  • 1j71 - PRO + polypeptide inhibitor – Candida tropicalis
    
  • 1ize - AoPRO + polypeptide-statin inhibitor
    
  • 1wkr - PRO + polypeptide-statin inhibitor – Irpex lacteus
• Cysteine Proteinase
  • 2hrv – PRO 2A – human rhinovirus
• Serine Proteinase
  • 1s2n, 1sh7 – PRO – Vibrio
    
  • 3s9a, 3s9b – RvPRO – Siamese Russell’s viper
    
  • 3s9c, 3sbk – RvPRO + human factor V polypeptide
    
  • 1ga1, 1ga4, 1ga6, 1nlu – PsPRO + iodotyrostatin fragment – Pseudomonas
    
  • 1kdv, 1kdy, 1kdz, 1ke1, 1ke2 - PsPRO + polypeptide inhibitor