Sandbox Reserved 1053

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This Sandbox is Reserved from 02/09/2015, through 05/31/2016 for use in the course "CH462: Biochemistry 2" taught by Geoffrey C. Hoops at the Butler University. This reservation includes Sandbox Reserved 1051 through Sandbox Reserved 1080.
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BackgroundBackground

The antigen 85 (ag85) complex in Mycobacterium tuberculosis is composed of three intracellular membrane proteins: ag85A, B, and C. The ag85 complex is a major component of the cell wall, with each protein catalyzing the transfer of important cell wall constituents into the membrane. [1] Ag85C is of particular interest due to its transfer of mycolic acids, which are major components in determining cell wall integrity. By targeting this mycoloyltransferase activity, inhibition of ag85C offers potential for cell wall disruption and subsequent antibiotic targeting for normally drug-resistant mycotaberia tuberculosis. [2]

StructureStructure


General Structure

Antigen 85C was crystallized in its dimeric form [3] are composed of multiple alpha helices with one interwoven beta sheet.

Enzymatic Activity

The mycolyltransferase activity of Ag85C is important to maintain cell wall integrity.

Methods of Inhibition

Inhibitors

Mutations

Structural Changes

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Antigen 85C in Mycobacterium Tuberculosis

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ReferencesReferences

  1. ↑ Ronning DR, Klabunde T, Besra GS, Vissa VD, Belisle JT, Sacchettini JC. Crystal structure of the secreted form of antigen 85C reveals potential targets for mycobacterial drugs and vaccines. Nat Struct Biol. 2000 Feb;7(2):141-6. PMID:10655617 doi:10.1038/72413
  2. ↑ Jackson M, Raynaud C, Laneelle MA, Guilhot C, Laurent-Winter C, Ensergueix D, Gicquel B, Daffe M. Inactivation of the antigen 85C gene profoundly affects the mycolate content and alters the permeability of the Mycobacterium tuberculosis cell envelope. Mol Microbiol. 1999 Mar;31(5):1573-87. PMID:10200974
  3. ↑ Favrot L, Lajiness DH, Ronning DR. Inactivation of the Mycobacterium tuberculosis Antigen 85 complex by covalent, allosteric inhibitors. J Biol Chem. 2014 Jul 14. pii: jbc.M114.582445. PMID:25028518 doi:http://dx.doi.org/10.1074/jbc.M114.582445

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA, Ben Zercher, Geoffrey C. Hoops, Katelyn Baumer, Mary Liggett, Jakob Jozwiakowski
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