2fxl
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| , resolution 1.76Å | |||||||
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| Ligands: | |||||||
| Gene: | uaZ, uox (Aspergillus flavus) | ||||||
| Activity: | Urate oxidase, with EC number 1.7.3.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Urate oxidase from aspergillus flavus complexed with allantoin
OverviewOverview
Urate oxidase from Aspergillus flavus catalyzes the degradation of uric acid to [S]-allantoin through 5-hydroxyisourate as a metastable intermediate. The second degradation step is thought either catalyzed by another specific enzyme, or spontaneous. The structure of the enzyme was known at high resolution by X-ray diffraction of I222 crystals complexed with a purine-type inhibitor (8-azaxanthin). Analyzing the X-ray structure of urate oxidase treated with an excess of urate, the natural substrate, shows unexpectedly that the active site recaptures [S]-allantoin from the racemic end product of a second degradation step.
About this StructureAbout this Structure
2FXL is a Single protein structure of sequence from Aspergillus flavus. Full crystallographic information is available from OCA.
ReferenceReference
Recapture of [S]-allantoin, the product of the two-step degradation of uric acid, by urate oxidase., Gabison L, Chiadmi M, Colloc'h N, Castro B, El Hajji M, Prange T, FEBS Lett. 2006 Apr 3;580(8):2087-91. Epub 2006 Mar 10. PMID:16545381
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