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N-TERMINAL LEUCINE-REPEAT REGION OF HEPATITIS DELTA ANTIGENN-TERMINAL LEUCINE-REPEAT REGION OF HEPATITIS DELTA ANTIGEN
Structural highlights
Publication Abstract from PubMedHepatitis delta virus (HDV) is a satellite virus of the hepatitis B virus (HBV) which provides the surface antigen for the viral coat. The RNA genome of HDV encodes two proteins: the small delta antigen and the large delta antigen. The two proteins resemble each other except for the presence of an additional 19 amino acids at the C terminus of the latter species. We have found that the N-terminal leucine-repeat region of hepatitis delta antigen (HDAg) binds to the autolytic domain of HDV genomic RNA and attenuates its autolytic activity. A 27-residue polypeptide corresponding to residues 24-50 of HDAg, designated dAg(24-50), was synthesized, and its solution structure was found to be an alpha-helix by circular dichroism and (1)H-nuclear magnetic resonance (NMR) techniques. Binding affinity of dAg(24-50) with HDV genomic RNA was found to increase with its alpha-helical content, and it was further confirmed by modifying its N- and C-terminal groups. Furthermore, the absence of RNA binding activity in the mutant peptides, dAgM(24-50am) and dAgM(Ac24-50am), in which Lys38, Lys39, and Lys40 were changed to Glu, indicates a possible involvement of these residues in their binding activity. Structural knowledge of the N-terminal leucine-repeat region of HDAg thus provides a molecular basis for the understanding of its role in the interaction with RNA. Proteins 1999;37:121-129. Solution structure and RNA-binding activity of the N-terminal leucine-repeat region of hepatitis delta antigen.,Lin IJ, Lou YC, Pai MT, Wu HN, Cheng JW Proteins. 1999 Oct 1;37(1):121-9. PMID:10451556[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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