1t1b
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| , resolution 1.60Å | |||||||
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| Ligands: | |||||||
| Gene: | PYP (Halorhodospira halophila) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Late intermediate IL2 from time-resolved crystallography of the E46Q mutant of PYP
OverviewOverview
In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational change in the protein associated with differential signaling activity, as it executes a reversible photocycle. Time-resolved Laue crystallography allows structural snapshots (as short as 150 ps) of high crystallographic resolution (approximately 1.6 A) to be taken of a protein as it functions. Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns-100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion. A clear structural progression is visible in these intermediates, in which a signal generated at the chromophore propagates through a distinct structural pathway of conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore.
About this StructureAbout this Structure
1T1B is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.
ReferenceReference
A structural pathway for signaling in the E46Q mutant of photoactive yellow protein., Rajagopal S, Anderson S, Srajer V, Schmidt M, Pahl R, Moffat K, Structure. 2005 Jan;13(1):55-63. PMID:15642261
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