2bpi
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STUCTURE OF IRON DEPENDENT SUPEROXIDE DISMUTASE FROM P. FALCIPARUM.
OverviewOverview
BACKGROUND: Superoxide dismutases (SODs) are important enzymes in defence, against oxidative stress. In Plasmodium falciparum, they may be expected, to have special significance since part of the parasite life cycle is, spent in red blood cells where the formation of reactive oxygen species is, likely to be promoted by the products of haemoglobin breakdown. Thus, inhibitors of P. falciparum SODs have potential as anti-malarial, compounds. As a step towards their development we have determined the, crystal structure of the parasite's cytosolic iron superoxide dismutase., RESULTS: The cytosolic iron superoxide dismutase from P. falciparum, (PfFeSOD) has been overexpressed in E. coli in a catalytically active, form. Its crystal structure has been solved by molecular replacement and, refined against data extending to 2.5 A resolution. The structure reveals, a two-domain organisation and an iron centre in which the metal is, coordinated by three histidines, an aspartate and a solvent molecule., Consistent with ultracentrifugation analysis the enzyme is a dimer in, which a hydrogen bonding lattice links the two active centres. CONCLUSION:, The tertiary structure of PfFeSOD is very similar to those of a number of, other iron-and manganese-dependent superoxide dismutases, moreover the, active site residues are conserved suggesting a common mechanism of, action. Comparison of the dimer interfaces of PfFeSOD with the human, manganese-dependent superoxide dismutase reveals a number of differences, which may underpin the design of parasite-selective superoxide dismutase, inhibitors.
About this StructureAbout this Structure
2BPI is a Single protein structure of sequence from Plasmodium falciparum with FE as ligand. Active as Superoxide dismutase, with EC number 1.15.1.1 Structure known Active Site: AC1. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of superoxide dismutase from Plasmodium falciparum., Boucher IW, Brzozowski AM, Brannigan JA, Schnick C, Smith DJ, Kyes SA, Wilkinson AJ, BMC Struct Biol. 2006 Oct 4;6:20. PMID:17020617
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