3r3q
Crystal structure of the yeast Vps23 UEV domainCrystal structure of the yeast Vps23 UEV domain
Structural highlights
Publication Abstract from PubMedThe ESCRT-0 and ESCRT-I complexes coordinate the clustering of ubiquitinated cargo with intralumenal budding of the endosomal membrane, two essential steps in vacuolar/lysosomal protein sorting from yeast to humans. The 1.85-A crystal structure of interacting regions of the yeast ESCRT-0 and ESCRT-I complexes reveals that PSDP motifs of the Vps27 ESCRT-0 subunit bind to a novel electropositive N-terminal site on the UEV domain of the ESCRT-I subunit Vps23 centred on Trp16. This novel site is completely different from the C-terminal part of the human UEV domain that binds to P(S/T)AP motifs of human ESCRT-0 and HIV-1 Gag. Disruption of the novel PSDP-binding site eliminates the interaction in vitro and blocks enrichment of Vps23 in endosome-related class E compartments in yeast cells. However, this site is non-essential for sorting of the ESCRT cargo Cps1. Taken together, these results show how a conserved motif/domain pair can evolve to use strikingly different binding modes in different organisms. Structural basis for endosomal recruitment of ESCRT-I by ESCRT-0 in yeast.,Ren X, Hurley JH EMBO J. 2011 Apr 19. PMID:21505419[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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