4gcw

Crystal structure of RNase Z in complex with precursor tRNA(Thr)Crystal structure of RNase Z in complex with precursor tRNA(Thr)

Structural highlights 4gcw is a 2 chain structure with sequence from Bacsu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: , NonStd Res: , Related: 1y44, 2fk6 Gene: BSU23840, rnz, yqjK (BACSU) Activity: Ribonuclease Z, with EC number 3.1.26.11 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Ribonuclease (RNase) Z is involved in the maturation of the 3' ends of transfer RNAs (tRNAs) in all three kingdoms of life. To prevent futile cycles of CCA addition and removal, eukaryotic RNase Z discriminates against mature tRNAs bearing a CCA motif, with the first cytosine residue (C74) being the key antideterminant. Here, we show that, remarkably, the B. subtilis enzyme does not discriminate against cytosine in position 74, but rather is highly stimulated by uracil in this location. Consistent with this observation, the vast majority of B. subtilis tRNA precursor substrates of RNase Z naturally contain U74. Those tRNA precursors with a uracil further downstream are also substrates for RNase Z, but are matured in a two-step endo/exonuclease reaction. We solved the first crystal structure of B. subtilis RNase Z bound to a tRNA(Thr) precursor with U74 and show that the enzyme has a specific binding pocket for this nucleotide. Activation of tRNA maturation by downstream uracil residues in B. subtilis.,Pellegrini O, Li de la Sierra-Gallay I, Piton J, Gilet L, Condon C Structure. 2012 Oct 10;20(10):1769-77. doi: 10.1016/j.str.2012.08.002. Epub 2012 , Aug 30. PMID:22940585 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribonuclease User:Jaime.Prilusky/Test/tree

spinqualitylabelsall models 4gcw, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image