3os1

PFV target capture complex (TCC) at 2.97 A resolutionPFV target capture complex (TCC) at 2.97 A resolution

Structural highlights 3os1 is a 5 chain structure with sequence from Human spumaretrovirus. The March 2011 RCSB PDB Molecule of the Month feature on Integrase by David Goodsell is 10.2210/rcsb_pdb/mom_2011_3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: , , NonStd Res: Related: 3l2r, 3os0, 3os2 Gene: pol (Human spumaretrovirus) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed To establish productive infection, a retrovirus must insert a DNA replica of its genome into host cell chromosomal DNA. This process is operated by the intasome, a nucleoprotein complex composed of an integrase tetramer (IN) assembled on the viral DNA ends. The intasome engages chromosomal DNA within a target capture complex to carry out strand transfer, irreversibly joining the viral and cellular DNA molecules. Although several intasome/transpososome structures from the DDE(D) recombinase superfamily have been reported, the mechanics of target DNA capture and strand transfer by these enzymes remained unclear. Here we report crystal structures of the intasome from prototype foamy virus in complex with target DNA, elucidating the pre-integration target DNA capture and post-catalytic strand transfer intermediates of the retroviral integration process. The cleft between IN dimers within the intasome accommodates chromosomal DNA in a severely bent conformation, allowing widely spaced IN active sites to access the scissile phosphodiester bonds. Our results resolve the structural basis for retroviral DNA integration and provide a framework for the design of INs with altered target sequences. The mechanism of retroviral integration from X-ray structures of its key intermediates.,Maertens GN, Hare S, Cherepanov P Nature. 2010 Nov 11;468(7321):326-9. PMID:21068843 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Retroviral Integrase

spinqualitylabelsall models 3os1, resolution 2.97Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image