3qx8

Crystal structure of MID domain from hAGO2 in complex with m7GpppGCrystal structure of MID domain from hAGO2 in complex with m7GpppG

Structural highlights 3qx8 is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Related: 3luc Gene: EIF2C2, AGO2 (Homo sapiens) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed In RNA silencing, microRNA (miRNA)-mediated translational repression occurs through mechanisms that do not invoke messenger-RNA (mRNA) target cleavage by Argonaute proteins. The nature of these mechanisms is unclear, but several recent studies have proposed that a direct interaction between the mRNA-cap and the middle (MID) domain of Argonautes is involved. Here, we present crystallographic and NMR data demonstrating that cap analogues do not bind significantly to the isolated MID domain of human Argonaute 2 (hAGO2) and are found in the miRNA 5'-nucleotide binding site in an implausible binding mode. Additionally, in vitro pull-down experiments with full-length hAGO2 indicate that the interaction with cap analogues is nonspecific. Structural analysis of 5'-mRNA-cap interactions with the human AGO2 MID domain.,Frank F, Fabian MR, Stepinski J, Jemielity J, Darzynkiewicz E, Sonenberg N, Nagar B EMBO Rep. 2011 Apr 8. PMID:21475248 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Argonaute Eukaryotic initiation factor

spinqualitylabelsall models 3qx8, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image