2uvu

File:2uvu.gif

spinqualitylabelsall models 2uvu, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURES OF MUTANT DPO4 DNA POLYMERASES WITH 8-OXOG CONTAINING DNA TEMPLATE-PRIMER CONSTRUCTS

OverviewOverview

Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) has been shown to, catalyze bypass of 7,8-dihydro-8-oxodeoxyguanosine (8-oxoG) in a highly, efficient and relatively accurate manner. Crystal structures have revealed, a potential role for Arg(332) in stabilizing the anti conformation of the, 8-oxoG template base by means of a hydrogen bond or ion-dipole pair, which, results in an increased enzymatic efficiency for dCTP insertion and makes, formation of a Hoogsteen pair between 8-oxoG and dATP less favorable., Site-directed mutagenesis was used to replace Arg(332) with Ala, Glu, Leu, or His in order to probe the importance of Arg(332) in accurate and, efficient bypass of 8-oxoG. The double mutant Ala(331)Ala(332) was also, prepared to address the contribution of Arg(331). Transientstate ... [(full description)]

About this StructureAbout this Structure

2UVU is a [Single protein] structure of sequence from [Sulfolobus solfataricus] with CA and DGT as [ligands]. Active as [DNA-directed DNA polymerase], with EC number [2.7.7.7]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

ReferenceReference

Hydrogen bonding of 7,8-dihydro-8-oxodeoxyguanosine with a charged residue in the little finger domain determines miscoding events in Sulfolobus solfataricus DNA polymerase Dpo4., Eoff RL, Irimia A, Angel KC, Egli M, Guengerich FP, J Biol Chem. 2007 Jul 6;282(27):19831-43. Epub 2007 Apr 27. PMID:17468100

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