1jf9
Crystal Structure of selenocysteine lyase
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| , resolution 2.Å | |||||||
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| Ligands: | |||||||
| Gene: | NIFS/CSDB (Escherichia coli) | ||||||
| Activity: | Selenocysteine lyase, with EC number 4.4.1.16 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
OverviewOverview
The Escherichia coli NifS CsdB protein is a member of the homodimeric pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes are capable of decomposing cysteine or selenocysteine into L-alanine and sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity for L-selenocysteine in comparison to l-cysteine, suggesting a role for this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli NifS CsdB reveals a high-resolution view of the active site of this enzyme in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and perselenide intermediates during catalysis, a necessary intermediate in the formation of sulfur and selenium containing metabolites.
About this StructureAbout this Structure
1JF9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:11827487
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