2jwo

A PHD finger motif in the C-terminus of RAG2 modulates recombination activityA PHD finger motif in the C-terminus of RAG2 modulates recombination activity

Structural highlights 2jwo is a 1 chain structure with sequence from Mus musculus. This structure supersedes the now removed PDB entry 2a23. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Gene: Rag2, Rag-2 (Mus musculus) Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The RAG1 and RAG2 proteins catalyze V(D)J recombination and are essential for generation of the diverse repertoire of antigen receptor genes and effective immune responses. RAG2 is composed of a "core" domain that is required for the recombination reaction and a C-terminal nonessential or "non-core" region. Recent evidence has emerged arguing that the non-core region plays a critical regulatory role in the recombination reaction, and mutations in this region have been identified in patients with immunodeficiencies. Here we present the first structural data for the RAG2 protein, using NMR spectroscopy to demonstrate that the C terminus of RAG2 contains a noncanonical PHD finger. All of the non-core mutations of RAG2 that are implicated in the development of immunodeficiencies are located within the PHD finger, at either zinc-coordinating residues or residues adjacent to an alpha-helix on the surface of the domain that participates in binding to the signaling molecules, phosphoinositides. Functional analysis of disease and phosphoinositide-binding mutations reveals novel intramolecular interactions within the non-core region and suggests that the PHD finger adopts two distinct states. We propose a model in which the equilibrium between these states modulates recombination activity. Together, these data identify the PHD finger as a novel and functionally important domain of RAG2. A PHD finger motif in the C terminus of RAG2 modulates recombination activity.,Elkin SK, Ivanov D, Ewalt M, Ferguson CG, Hyberts SG, Sun ZY, Prestwich GD, Yuan J, Wagner G, Oettinger MA, Gozani OP J Biol Chem. 2005 Aug 5;280(31):28701-10. Epub 2005 Jun 17. PMID:15964836 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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