2aak

UBIQUITIN CONJUGATING ENZYME FROM ARABIDOPSIS THALIANAUBIQUITIN CONJUGATING ENZYME FROM ARABIDOPSIS THALIANA

Structural highlights 2aak is a 1 chain structure with sequence from Arabidopsis thaliana. This structure supersedes the now removed PDB entry 1aak. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Activity: Ubiquitin--protein ligase, with EC number 6.3.2.19 Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The x-ray crystal structure of a recombinant ubiquitin-conjugating enzyme (E2) encoded by the UBC1 gene of the plant Arabidopsis thaliana has been determined with the use of multiple isomorphous replacement techniques and refined at 2.4-A resolution by simulated annealing and restrained least-squares. This E2 is an alpha/beta protein, with four alpha-helices and a four-stranded antiparallel beta-sheet. The NH2 and COOH termini, which may be important for interaction with other enzymes and substrates in the ubiquitin-conjugation pathway, are on the opposite side of the molecule from the cysteine residue that binds to the COOH terminus of ubiquitin. This structure should now allow for the rational analysis of E2 function by in vitro mutagenesis and facilitate the effective design of E2s with unique specificities or catalytic functions. Three-dimensional structure of a ubiquitin-conjugating enzyme (E2).,Cook WJ, Jeffrey LC, Sullivan ML, Vierstra RD J Biol Chem. 1992 Jul 25;267(21):15116-21. PMID:1321826 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also PDB identification code Ubiquitin conjugating enzyme

spinqualitylabelsall models 2aak, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image