3h4d

Ternary complex of human DNA polymerase iota with template U/T and incoming dGTPTernary complex of human DNA polymerase iota with template U/T and incoming dGTP

Structural highlights 3h4d is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: , NonStd Res: , Related: 3h40, 3h4b Gene: POLI, RAD30B (Homo sapiens) Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7 Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Human DNA polymerase-iota (Poliota) incorporates correct nucleotides opposite template purines with a much higher efficiency and fidelity than opposite template pyrimidines. In fact, the fidelity opposite template T is so poor that Poliota inserts an incorrect dGTP approximately 10 times better than it inserts the correct dATP. We determine here how a template T/U is accommodated in the Poliota active site and why a G is incorporated more efficiently than an A. We show that in the absence of incoming dATP or dGTP (binary complex), template T/U exists in both syn and anti conformations, but in the presence of dATP or dGTP (ternary complexes), template T/U is predominantly in the anti conformation. We also show that dATP and dGTP insert differently opposite template T/U, and that the basis of selection of dGTP over dATP is a hydrogen bond between the N2 amino group of dGTP and Gln59 of Poliota. Replication across template T/U by human DNA polymerase-iota.,Jain R, Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK Structure. 2009 Jul 15;17(7):974-80. PMID:19604477 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also DNA polymerase

spinqualitylabelsall models 3h4d, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image