1ex4

HIV-1 INTEGRASE CATALYTIC CORE AND C-TERMINAL DOMAINHIV-1 INTEGRASE CATALYTIC CORE AND C-TERMINAL DOMAIN

Structural highlights 1ex4 is a 2 chain structure with sequence from Human immunodeficiency virus 1. The March 2011 RCSB PDB Molecule of the Month feature on Integrase by David Goodsell is 10.2210/rcsb_pdb/mom_2011_3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Insolubility of full-length HIV-1 integrase (IN) limited previous structure analyses to individual domains. By introducing five point mutations, we engineered a more soluble IN that allowed us to generate multidomain HIV-1 IN crystals. The first multidomain HIV-1 IN structure is reported. It incorporates the catalytic core and C-terminal domains (residues 52-288). The structure resolved to 2.8 A is a Y-shaped dimer. Within the dimer, the catalytic core domains form the only dimer interface, and the C-terminal domains are located 55 A apart. A 26-aa alpha-helix, alpha6, links the C-terminal domain to the catalytic core. A kink in one of the two alpha6 helices occurs near a known proteolytic site, suggesting that it may act as a flexible elbow to reorient the domains during the integration process. Two proteins that bind DNA in a sequence-independent manner are structurally homologous to the HIV-1 IN C-terminal domain, suggesting a similar protein-DNA interaction in which the IN C-terminal domain may serve to bind, bend, and orient viral DNA during integration. A strip of positively charged amino acids contributed by both monomers emerges from each active site of the dimer, suggesting a minimally dimeric platform for binding each viral DNA end. The crystal structure of the isolated catalytic core domain (residues 52-210), independently determined at 1.6-A resolution, is identical to the core domain within the two-domain 52-288 structure. Crystal structure of the HIV-1 integrase catalytic core and C-terminal domains: a model for viral DNA binding.,Chen JC, Krucinski J, Miercke LJ, Finer-Moore JS, Tang AH, Leavitt AD, Stroud RM Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8233-8. PMID:10890912 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Retroviral Integrase

spinqualitylabelsall models 1ex4, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image