1dzf
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| , resolution 1.9Å | |||||||
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| Gene: | YBR154C OR YBR1204 (Saccharomyces cerevisiae) | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RPB5 FROM S.CEREVISIAE
OverviewOverview
Eukaryotic nuclei contain three different types of RNA polymerases (RNAPs), each consisting of 12-18 different subunits. The evolutionarily highly conserved RNAP subunit RPB5 is shared by all three enzymes and therefore represents a key structural/functional component of all eukaryotic RNAPs. Here we present the crystal structure of the RPB5 subunit from Saccharomyces cerevisiae. The bipartite structure includes a eukaryote-specific N-terminal domain and a C-terminal domain resembling the archaeal RNAP subunit H. RPB5 has been implicated in direct protein-protein contacts with transcription factor IIB, one of the components of the RNAP(II) basal transcriptional machinery, and gene-specific activator proteins, such as the hepatitis B virus transactivator protein X. The experimentally mapped regions of RPB5 involved in these interactions correspond to distinct and surface-exposed alpha-helical structures.
About this StructureAbout this Structure
1DZF is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of RPB5, a universal eukaryotic RNA polymerase subunit and transcription factor interaction target., Todone F, Weinzierl RO, Brick P, Onesti S, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6306-10. PMID:10841537
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