Aspartate carbamoyltransferase

<StructureSection load='1raa' size='340' side='right' caption='Structure of E. coli aspartate carbamoyltransferase catalytic and regulatory subunits complex with CTP and Zn+2 (grey) (PDB code 1raa).tructure' scene=>

FunctionFunction

Aspartate carbamoyltransferase (ATC) is part of the pyrimidine biosynthesis pathway. ATC catalyzes the condensation of aspartate and carbamoyl phosphate to N-carbamyl-L-aspartate and phosphate. ATC is composed of 2 trimers of catalytic subunits (C) and 3 dimers of regulatory subunits (R). The catalytic subunit contains an aspartate-binding domain and a carbamoyl-phosphate-binding domain. The regulatory subunit contains a nucleotide effectors-binding domain and a zinc domain. The Zn atom is essential for the association of the subunits. Binding of the substrate to the catalytic subunits results in a high-affinity state while binding of CTP to the regulatory subunit results in a low-affinity state. Malate and phosphonoacetyl-L-aspartate are inhibitors of ATC.

3D structures of aspartate carbamoyltransferase3D structures of aspartate carbamoyltransferase

Updated on 19-June-2014

2atc, 3at1, 6at1, 1pg5, 3d7s – EcATC C+R subunits – Escherichia coli
9atc – EcATC C (mutant) + R (mutant) subunits
1ezz, 4e2f – EcATC C (mutant) + R subunits
2qg9, 2qgf – EcATC C + R (mutant) subunits
2be7 – ATC C (mutant) + R subunits – Moritella profunda

ATC binary complexATC binary complex

1at1 – EcATC C+R subunits + malonate
1sku – EcATC C (mutant) +R subunits + malonate
1r0b – EcATC C+R subunits + citrate
1r0c – EcATC C+R subunits + N-carbamoyl-L-aspartate + phosphate
1xjw – EcATC C (mutant) +R subunits + phosphonacetyl-L-aspartate
1sku – EcATC C (mutant) +R subunits + phosphonoacetamide
1tu0 – EcATC C+R subunits + maltose
4at1 – EcATC C+R subunits + ATP
5at1, 1raa, 1rab, 1rac, 1rad, 1rae, 1raf, 1rag, 1rah, 1rai, 1za1, 4fyw – EcATC C+R subunits + CTP
8atc, 1acm, 1d09, 1q95 – EcATC C+R subunits + phosphonacetyl aspartate
1f1b, 1i5o, 1tth – EcATC C (mutant) +R subunits + phosphonacetyl aspartate
2h3e – EcATC C+R subunits + phosphonacetyl asparagine
1nbe – EcATC C (mutant) + R (mutant) subunits + malate
2a0f – EcATC C (mutant) +R subunits + phosphonoacetamide
3mpu – EcATC C (mutant) +R subunits + phosphate
2be9 – ATC C+R subunits + CTP – Sulfolobus acidocaldarius
2yww – ATC R subunit + ATP – Methanocaldococcus jannaschii

ATC ternary complexATC ternary complex

7at1 – EcATC C+R subunits + ATP + maltose
8at1 – EcATC C+R subunits + CTP + maltose
4fyv – EcATC C+R subunits + dCTP + phosphate
4fyx – EcATC C+R subunits + dCTP + UTP
4fyy – EcATC C+R subunits + CTP + UTP
1za2 – EcATC C+R subunits + CTP + phosphoric monoformamide ester
2air – EcATC C+R subunits + L-alanosine + phosphoric monoformamide ester
2fzc, 2fzg – EcATC C+R subunits + CTP + phosphonic acid derivative
2fzk – EcATC C+R subunits + CTP + phosphonacetyl benzoate
4f04, 4kgv, 4kgx, 4kgz, 4kh0 – EcATC C+R subunits + nucleotide + phosphonacetyl aspartate
2h3e – EcATC C+R subunits + phosphonacetyl asparagine + malate

ATC quaternary complexATC quaternary complex

1tug – EcATC C (mutant) +R subunits + phosphate + aspartate + phosphonoacetamide
1tug – EcATC C (mutant) +R subunits + CTP + malonate + phosphonoacetamide
4kh1 – EcATC C+R subunits + UTP + CTP + phosphate + phosphonacetyl aspartate