2cck
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CRYSTAL STRUCTURE OF UNLIGANDED S. AUREUS THYMIDYLATE KINASE
OverviewOverview
Methicillin-resistant Staphylococcus aureus (MRSA) poses a major threat to, human health, particularly through hospital acquired infection. The spread, of MRSA means that novel targets are required to develop potential, inhibitors to combat infections caused by such drug-resistant bacteria., Thymidylate kinase (TMK) is attractive as an antibacterial target as it is, essential for providing components for DNA synthesis. Here, we report, crystal structures of unliganded and thymidylate-bound forms of S. aureus, thymidylate kinase (SaTMK). His-tagged and untagged SaTMK crystallize with, differing lattice packing and show variations in conformational states for, unliganded and thymidylate (TMP) bound forms. In addition to open and, closed forms of SaTMK, an intermediate conformation in TMP ... [(full description)]
About this StructureAbout this Structure
2CCK is a [Single protein] structure of sequence from [Staphylococcus aureus] with CL and EDO as [ligands]. Active as [dTMP kinase], with EC number [2.7.4.9]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Structures of S. aureus thymidylate kinase reveal an atypical active site configuration and an intermediate conformational state upon substrate binding., Kotaka M, Dhaliwal B, Ren J, Nichols CE, Angell R, Lockyer M, Hawkins AR, Stammers DK, Protein Sci. 2006 Apr;15(4):774-84. Epub 2006 Mar 7. PMID:16522804
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