4g0q

Crystal structure of Arabidopsis thaliana AGO1 MID domain in complex with CMPCrystal structure of Arabidopsis thaliana AGO1 MID domain in complex with CMP

Structural highlights 4g0q is a 1 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Related: 4g0m, 4g0o, 4g0p, 4g0x, 4g0y, 4g0z Gene: AGO1, At1g48410, F11A17.3, T1N15.2 (Arabidopsis thaliana) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The 5'-nucleotide of small RNAs associates directly with the MID domain of Argonaute (AGO) proteins. In humans, the identity of the 5'-base is sensed by the MID domain nucleotide specificity loop and regulates the integrity of miRNAs. In Arabidopsis thaliana, the 5'-nucleotide also controls sorting of small RNAs into the appropriate member of the AGO family; however, the structural basis for this mechanism is unknown. Here, we present crystal structures of the MID domain from three Arabidopsis AGOs, AtAGO1, AtAGO2 and AtAGO5, and characterize their interactions with nucleoside monophosphates (NMPs). In AtAGOs, the nucleotide specificity loop also senses the identity of the 5'-nucleotide but uses more diverse modes of recognition owing to the greater complexity of small RNAs found in plants. Binding analyses of these interactions reveal a strong correlation between their affinities and evolutionary conservation. Arabidopsis Argonaute MID domains use their nucleotide specificity loop to sort small RNAs.,Frank F, Hauver J, Sonenberg N, Nagar B EMBO J. 2012 Jul 31. doi: 10.1038/emboj.2012.204. PMID:22850669 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Argonaute

spinqualitylabelsall models 4g0q, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image