1t5l
Crystal structure of the DNA repair protein UvrB point mutant Y96A revealing a novel fold for domain 2Crystal structure of the DNA repair protein UvrB point mutant Y96A revealing a novel fold for domain 2
Template:ABSTRACT PUBMED 15192705
FunctionFunction
[UVRB_BACCA] The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage (By similarity).[HAMAP-Rule:MF_00204]
About this StructureAbout this Structure
1t5l is a 2 chain structure with sequence from Bacillus caldotenax. Full crystallographic information is available from OCA.
See AlsoSee Also
ReferenceReference
- ↑ Truglio JJ, Croteau DL, Skorvaga M, DellaVecchia MJ, Theis K, Mandavilli BS, Van Houten B, Kisker C. Interactions between UvrA and UvrB: the role of UvrB's domain 2 in nucleotide excision repair. EMBO J. 2004 Jul 7;23(13):2498-509. Epub 2004 Jun 10. PMID:15192705 doi:10.1038/sj.emboj.7600263