EPSP synthase

Structure of EPSP SynthaseStructure of EPSP Synthase

5-enolpyruvylshikimate 3-phosphate (EPSP) synthase is a key enzyme for the biosynthesis of aromatic amino acids in plants and many microbes. Consequently, it is a target for drugs and herbicides. EPSP synthase catalyzes the addition of phosphoenol pyruvate (PEP) to shikimate-3-phosphate, generating 5-enolpyruvylshikimate-3-phosphate, which is a precursor for phenylalanine and tyrosine. The enzyme has , with the active site found in the interdomain cleft. There is a substantial structural change upon substrate binding, resulting in a . Glyphosate (also known as Roundup) occupies the binding site of the second substrate, phosphoenol pyruvate. 3D structures of EPSP synthase Updated on 05-May-2025 1eps – EcEPSP – Escherichia coli 1p88, 1p89 - EcEPSP N terminal - NMR 1rf5 - SpEPSP – Streptococcus pneumoniae 2bjb, 2o15 – MtEPSP – Mycobacterium tuberculosis 2gg4 – AgEPSP – Agrobacterium 3roi, 3tr1, 4gfp – CbEPSP – Coxiella burnetii 3ti2 - VcEPSP N terminal – Vibrio cholerae EPSP synthase binary complex 1g6t - EcEPSP + S3P 1mi4, 2qfq, 2qfs, 3fjx, 3fk0 - EcEPSP (mutant) + S3P 1q36 - EcEPSP (mutant) + S3P derivative 1x8r, 1x8t - EcEPSP + intermediate 2aa9 – EcEPSP + shikimate 2pq9 - EcEPSP + intermediate 1rf4 - SpEPSP + intermediate 2o0b, 2o0d - MtEPSP + S3P 2gg6 - AgEPSP + S3P 2pqb, 2pqc, 2pqd - AgEPSP + intermediate 2o0x - MtEPSP + intermediate 2o0z - MtEPSP + EPS 4egr - CbEPSP + phosphoenolpyruvate EPSP synthase ternary complex 1g6p - EcEPSP + S3P + glyphosate 2qft, 2qfu, 3fjz, 3fk1 - EcEPSP (mutant) + S3P + glyphosate 2aay - EcEPSP + shikimate + glyphosate 1rf6 - SpEPSP + S3P + glyphosate 2gga - AgEPSP + S3P + glyphosate 4ggd - AgEPSP (mutant) + S3P + glyphosate 2o0e - MtEPSP + S3P + phosphoenolpyruvate 3nvs - VcEPSP + S3P + glyphosate 3slh - CbEPSP + S3P + glyphosate

spinqualitylabelsall models Structure of EPSP synthase (PDB entry 1eps) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image