3a28

Crystal structure of L-2,3-butanediol dehydrogenaseCrystal structure of L-2,3-butanediol dehydrogenase

Template:ABSTRACT PUBMED 19941855

FunctionFunction

[BUDC_CORGT] Catalyzes the reversible reduction of (S)-acetoin to (S,S)-butane-2,3-diol (L-BD) in the presence of NADH. To a lesser extent, can also catalyze the irreversible reduction of diacetyl to (S)-acetoin. Cannot oxidize meso-BD, D-BD, 2-butanol, 1,2-propanediol, ethanol, acetol, 1,2-butanediol, 1,3-butanediol, n-butanol, and n-propanol. Cannot reduce (R)-acetoin, acetol, dihydroxyacetone and 2,4-pentanedione.[REFERENCE:1]^[1]

About this StructureAbout this Structure

3a28 is a 8 chain structure with sequence from "micrococcus_glutamicus"_kinoshita_et_al._1958 "micrococcus glutamicus" kinoshita et al. 1958. Full crystallographic information is available from OCA.

ReferenceReference

^{[xtra 1]}

↑ Otagiri M, Ui S, Takusagawa Y, Ohtsuki T, Kurisu G, Kusunoki M. Structural basis for chiral substrate recognition by two 2,3-butanediol dehydrogenases. FEBS Lett. 2010 Jan 4;584(1):219-23. Epub . PMID:19941855 doi:10.1016/j.febslet.2009.11.068

↑ Takusagawa Y, Otagiri M, Ui S, Ohtsuki T, Mimura A, Ohkuma M, Kudo T. Purification and characterization of L-2,3-butanediol dehydrogenase of Brevibacterium saccharolyticum C-1012 expressed in Escherichia coli. Biosci Biotechnol Biochem. 2001 Aug;65(8):1876-8. PMID:11577733

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

[2] Otagiri M, Ui S, Takusagawa Y, Ohtsuki T, Kurisu G, Kusunoki M. Structural basis for chiral substrate recognition by two 2,3-butanediol dehydrogenases. FEBS Lett. 2010 Jan 4;584(1):219-23. Epub . PMID:19941855 doi:10.1016/j.febslet.2009.11.068

[1] Takusagawa Y, Otagiri M, Ui S, Ohtsuki T, Mimura A, Ohkuma M, Kudo T. Purification and characterization of L-2,3-butanediol dehydrogenase of Brevibacterium saccharolyticum C-1012 expressed in Escherichia coli. Biosci Biotechnol Biochem. 2001 Aug;65(8):1876-8. PMID:11577733

[1]

[xtra 1]