Sandbox Reserved 813

This Sandbox is Reserved from 06/12/2018, through 30/06/2019 for use in the course "Structural Biology" taught by Bruno Kieffer at the University of Strasbourg, ESBS. This reservation includes Sandbox Reserved 1480 through Sandbox Reserved 1543.

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IntroductionIntroduction

The POU family come from the acronym of three transcription factors : Pit-1, Oct-1 and Oct-2 and Unc-86.

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Oct-4 structureOct-4 structure

There are two diffent domains in the Oct-4 sequence : the Pou-specific domain (POUs) located on the N-terminal subunit and the homeodomain located in the C-terminal subunit of the sequence.

Pou-specific domainPou-specific domain

The Pou-specific domain is located on the N-terminal site of the Oct-4 sequence and precisely from the 131th amino acid to the 205th. The POU-specific domain is able to bind autonomously the DNA with the binding consensus : [gAATAT(G/T)CA]. This is the thanks to a specific motif, the helix-turn-helix. At its name suggests, this pattern is composed by two α helix linked by an amino acids sequence which corresponds to the turn. The second helix is the most involved in the DNA binding. Indeed, this helix is able to carry out hydrogen bonds and Van der Waalts interactions with bases in the major groove of DNA. The first helix allows to stabilize the complex DNA-protein.

HomeodomainHomeodomain

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ReferencesReferences

POU-domain transcription factors: pou-er-ful developmental regulators. M G Rosenfeld Genes Dev. 1991 5: 897-907