4lk0
Crystal Structure Analysis of the E.coli holoenzyme/T7 Gp2 complexCrystal Structure Analysis of the E.coli holoenzyme/T7 Gp2 complex
Template:ABSTRACT PUBMED 24218560
FunctionFunction
[VRPI_BPT7] Bacterial RNA polymerase inhibitor, which is expressed in the late stage of lytic development, is also essential for continuous synthesis of phage DNA and functions in the packaging of the viral genome into the mature phage particle. [C9QUL2_ECOD1] Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits (By similarity).[HAMAP-Rule:MF_00366][SAAS:SAAS012293_004_017283] [C9QXI7_ECOD1] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).[HAMAP-Rule:MF_00059] [C5A0S8_ECOBW] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).[SAAS:SAAS012754_004_011999] [C9QV90_ECOD1] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).[HAMAP-Rule:MF_01321][RuleBase:RU000432] [RPOD_ECOLI] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium.
About this StructureAbout this Structure
4lk0 is a 14 chain structure with sequence from Bpt7, Ecobw, Ecod1 and Ecoli. Full crystallographic information is available from OCA.
ReferenceReference
- ↑ Bae B, Davis E, Brown D, Campbell EA, Wigneshweraraj S, Darst SA. Phage T7 Gp2 inhibition of Escherichia coli RNA polymerase involves misappropriation of sigma70 domain 1.1. Proc Natl Acad Sci U S A. 2013 Nov 11. PMID:24218560 doi:http://dx.doi.org/10.1073/pnas.1314576110