2jmc

OverviewOverview

Here we present the high-resolution NMR structure of a chimera (SPCp41) between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary structure mimics perfectly the interactions typically found in SH3-peptide complexes and is remarkably similar to that of the complex between the separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight binding between the ligand and SH3 part of the chimera. This chimera will serve as a tool for a deeper understanding of the relationship between structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an effective strategy for ligand design.

About this StructureAbout this Structure

2JMC is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

The high-resolution NMR structure of a single-chain chimeric protein mimicking a SH3-peptide complex., Candel AM, Conejero-Lara F, Martinez JC, van Nuland NA, Bruix M, FEBS Lett. 2007 Feb 20;581(4):687-92. Epub 2007 Jan 22. PMID:17275816

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