4lg2
Crystal structure of Reston Ebola virus VP35 RNA binding domain bound to 12-bp dsRNACrystal structure of Reston Ebola virus VP35 RNA binding domain bound to 12-bp dsRNA
FunctionFunction
[VP35_EBORR] Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (By similarity).
About this StructureAbout this Structure
4lg2 is a 8 chain structure with sequence from Reston ebolavirus - reston (1989). Full crystallographic information is available from OCA.
ReferenceReference
- ↑ Bale S, Julien JP, Bornholdt ZA, Krois AS, Wilson IA, Saphire EO. Ebolavirus VP35 also coats the backbone of dsRNA for interferon antagonism. J Virol. 2013 Jul 3. PMID:23824825 doi:10.1128/JVI.01452-13