1qmq
OPTICAL DETECTION OF CYTOCHROME P450 BY SENSITIZER-LINKED SUBSTRATES
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OverviewOverview
The ability to detect, characterize, and manipulate specific biomolecules, in complex media is critical for understanding metabolic processes., Particularly important targets are oxygenases (cytochromes P450) involved, in drug metabolism and many disease states, including liver and kidney, dysfunction, neurological disorders, and cancer. We have found that Ru, photosensitizers linked to P450 substrates specifically recognize, submicromolar cytochrome P450(cam) in the presence of other heme proteins., In the P450:Ru-substrate conjugates, energy transfer to the heme, dramatically accelerates the Ru-luminescence decay. The crystal structure, of a P450(cam):Ru-adamantyl complex reveals access to the active center, via a channel whose depth (Ru-Fe distance is 21 A) is virtually the same, as ... [(full description)]
About this StructureAbout this Structure
1QMQ is a [Single protein] structure of sequence from [Pseudomonas putida] with ACT, HEM and LRB as [ligands]. Active as [Camphor 5-monooxygenase], with EC number [1.14.15.1]. Structure known Active Site: HEM. Full crystallographic information is available from [OCA].
ReferenceReference
Optical detection of cytochrome P450 by sensitizer-linked substrates., Dmochowski IJ, Crane BR, Wilker JJ, Winkler JR, Gray HB, Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):12987-90. PMID:10557259
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