Barnase

Crystal Structure of Barnase 1bni

Barnase (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium Bacillus amyloliquefaciens. The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.

3D Structures of Barnase3D Structures of Barnase

3kch, 2f4y – BA cross-linked
2f56 - BA cross-linked + urea
2f5w - BA cross-linked + thiourea
2f5m - BA cross-linked + Bromo-ethanol
1fw7, 1bnr – BA - NMR
2kf3 – BA (mutant) – NMR
2kf4, 1b2x, 1a2p, 1bni, 1bnj – BA
1yvs – BA domain swapped
1b20, 1b21, 1b2z, 1bne, 1bnf, 1bng, 1brh, 1bri, 1brj, 1brk, 1bns, 1brg, 1bsa, 1bsb, 1bsc, 1bsd, 1bse, 1ban, 1bao – BA (mutant)
2kf5, 2kf6, 1brn, 1rnb – BA + polynucleotide

BA + BSTBA + BST

3da7 - BA (mutant) + BST
2za4, 1brs, 1bgs – BA + BST
1x1u, 1x1w, 1x1x, 1x1y, 1b27, 1b2u – BA + BST (mutant)
1b3s, 1b2s - BA (mutant) + BST (mutant)

2c4b – BA/MCOEETI (mutant)

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Michal Harel, Jaime Prilusky, Alexander Berchansky