1h8v
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THE X-RAY CRYSTAL STRUCTURE OF THE TRICHODERMA REESEI FAMILY 12 ENDOGLUCANASE 3, CEL12A, AT 1.9 A RESOLUTION
OverviewOverview
We present the three-dimensional structure of Trichoderma reesei, endoglucanase 3 (Cel12A), a small, 218 amino acid residue (24.5 kDa), neutral pI, glycoside hydrolase family 12 cellulase that lacks a, cellulose-binding module. The structure has been determined using X-ray, crystallography and refined to 1.9 A resolution. The asymmetric unit, consists of six non-crystallographic symmetry-related molecules that were, exploited to improve initial multiple isomorphous replacement phasing, and, subsequent structure refinement. The enzyme contains one disulfide bridge, and is glycosylated at Asp164 by a single N-acetyl glucosamine residue., The protein has the expected fold for a glycoside hydrolase clan-C family, 12 enzyme. It contains two beta-sheets, of six and nine strands, packed on, top ... [(full description)]
About this StructureAbout this Structure
1H8V is a [Single protein] structure of sequence from [Hypocrea jecorina] with NAG as [ligand]. Active as [Cellulase], with EC number [3.2.1.4]. Structure known Active Sites: CA1, CA2, CA3, CA4, CA5 and CA6. Full crystallographic information is available from [OCA].
ReferenceReference
The X-ray crystal structure of the Trichoderma reesei family 12 endoglucanase 3, Cel12A, at 1.9 A resolution., Sandgren M, Shaw A, Ropp TH, Wu S, Bott R, Cameron AD, Stahlberg J, Mitchinson C, Jones TA, J Mol Biol. 2001 Apr 27;308(2):295-310. PMID:11327768
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