1bb6
|
LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOTRIOSE
OverviewOverview
Two complexes between rainbow trout lysozyme (RBTL) and, 4-methylumbelliferyl chitobioside, 4MeU-(GlcNAc)2, and chitotrioside, 4MeU-(GlcNAc)3, were produced by co-crystallization and soaking, respectively, and the crystal structures were solved at 2.0 A resolution., The results show that 4-MeU-(GlcNAc)3 binds in subsites A-D and that, 4-MeU-(GlcNAc)2 binds in subsites B-D in the active-site cleft of RBTL., This agrees well with earlier crystallographic studies on the binding of, oligosaccharides of chitin to RBTL, which showed that (GlcNAc)3 binds to, sites B-D in RBTL and not to A-C as seen in the human and turkey egg-white, lysozymes. For both complexes the 4-MeU moiety in site D has diffuse, electron density and is flexible, as it is only bound to water molecules, and not to the ... [(full description)]
About this StructureAbout this Structure
1BB6 is a [Single protein] structure of sequence from [Oncorhynchus mykiss] with UMG as [ligand]. Active as [Lysozyme], with EC number [3.2.1.17]. Structure known Active Site: NUL. Full crystallographic information is available from [OCA].
ReferenceReference
Structural studies on the binding of 4-methylumbelliferone glycosides of chitin to rainbow trout lysozyme., Vollan VB, Hough E, Karlsen S, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):60-6. Epub 1999 Jan, 1. PMID:10089395
Page seeded by OCA on Tue Oct 30 14:53:50 2007