1ayk

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1ayk

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INHIBITOR-FREE CATALYTIC FRAGMENT OF HUMAN FIBROBLAST COLLAGENASE, NMR, 30 STRUCTURES

OverviewOverview

The high-resolution solution structure of the inhibitor-free catalytic, fragment of human fibroblast collagenase (MMP-1), a protein of 18.7 kDa, which is a member of the matrix metalloproteinase family, has been, determined using three-dimensional heteronuclear NMR spectroscopy. A total, of 30 structures were calculated by means of hybrid distance, geometry-simulated annealing using a total of 3333 experimental NMR, restraints, consisting of 2409 approximate interproton distance, restraints, 84 distance restraints for 42 backbone hydrogen bonds, 426, torsion angle restraints, 125 3JNH alpha restraints, 153 C alpha, restraints, and 136 C beta restraints. The atomic rms distribution about, the mean coordinate positions for the 30 structures for residues 7-137 and, 145-163 is 0.42 +/- 0.04 A ... [(full description)]

About this StructureAbout this Structure

1AYK is a [Single protein] structure of sequence from [Homo sapiens] with ZN and CA as [ligands]. Active as [Interstitial collagenase], with EC number [3.4.24.7]. Structure known Active Sites: CAB, ZNA and ZNB. Full crystallographic information is available from [OCA].

ReferenceReference

High-resolution solution structure of the inhibitor-free catalytic fragment of human fibroblast collagenase determined by multidimensional NMR., Moy FJ, Chanda PK, Cosmi S, Pisano MR, Urbano C, Wilhelm J, Powers R, Biochemistry. 1998 Feb 10;37(6):1495-504. PMID:9484219

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