1oc4
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LACTATE DEHYDROGENASE FROM PLASMODIUM BERGHEI
OverviewOverview
As Plasmodium rely extensively on homolactic fermentation for energy, production, Plasmodium falciparum lactate dehydrogenase (PfLDH)--the key, enzyme in this process--has previously been suggested as a novel target, for antimalarials. This enzyme has distinctive kinetic and structural, properties that distinguish it from its human homologues. In this study, we now describe the expression, kinetic characterisation and crystal, structure determination of the LDH from Plasmodium berghei. This enzyme is, seen to have a similar kinetic profile to its P. falciparum counterpart, exhibiting the characteristic lack of substrate inhibition that, distinguishes plasmodial from human LDHs. The crystal structure of P., berghei lactate dehydrogenase (PbLDH) shows a very similar active site, arrangement ... [(full description)]
About this StructureAbout this Structure
1OC4 is a [Single protein] structure of sequence from [Plasmodium berghei] with NAD, OXM and GOL as [ligands]. Active as [L-lactate dehydrogenase], with EC number [1.1.1.27]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Crystal structure of Plasmodium berghei lactate dehydrogenase indicates the unique structural differences of these enzymes are shared across the Plasmodium genus., Winter VJ, Cameron A, Tranter R, Sessions RB, Brady RL, Mol Biochem Parasitol. 2003 Sep;131(1):1-10. PMID:12967707
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