CcNiR
Your Heading Here ('ccNiR')Your Heading Here ('ccNiR')
| Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction [1]
| ||||||||||
Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.
The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at a pentahemic subunit of 61 kDa which is strongly bound to its physiological electron donor, a smaller hydrophobic polypeptide tetrahemic of 19 kDa, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).
- ↑ Silveira CM, Gomes SP, Araujo AN, Montenegro MC, Todorovic S, Viana AS, Silva RJ, Moura JJ, Almeida MG. An efficient non-mediated amperometric biosensor for nitrite determination. Biosens Bioelectron. 2010 May 15;25(9):2026-32. Epub 2010 Feb 6. PMID:20188536 doi:10.1016/j.bios.2010.01.031