2bt6
|
RU(BPY)2(MBPY)-MODIFIED BOVINE ADRENODOXIN
OverviewOverview
Bovine adrenodoxin (Adx) plays an important role in the electron-transfer, process in the mitochondrial steroid hydroxylase system of the bovine, adrenal cortex. Using electron paramagnetic resonance (EPR) spectroscopy, we showed that photoreduction of the [2Fe-2S] cluster of Adx via, (4'-methyl-2,2'-bipyridine)bis(2,2'-bipyridine)ruthenium(II), [Ru(bpy)2(mbpy)] covalently attached to the protein surface can be used as, a new approach to probe the "shuttle" hypothesis for the electron transfer, by Adx. The 1.5 A resolution crystal structure of a 1:1, Ru(bpy)2(mbpy)-Adx(1-108) complex reveals the site of modification, Cys95, and allows to predict the possible intramolecular electron-transfer, pathways within the complex. Photoreduction of uncoupled Adx, mutant, Adx(1-108), and ... [(full description)]
About this StructureAbout this Structure
2BT6 is a [Single protein] structure of sequence from [Bos taurus] with MG, FES and RUA as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Light-induced reduction of bovine adrenodoxin via the covalently bound ruthenium(II) bipyridyl complex: intramolecular electron transfer and crystal structure., Halavaty A, Muller JJ, Contzen J, Jung C, Hannemann F, Bernhardt R, Galander M, Lendzian F, Heinemann U, Biochemistry. 2006 Jan 24;45(3):709-18. PMID:16411746
Page seeded by OCA on Tue Oct 30 14:23:02 2007
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Bos taurus
- Single protein
- Bernhardt, R.
- Contzen, J.
- Galander, M.
- Halavaty, A.
- Hannemann, F.
- Heinemann, U.
- Jung, C.
- Lendzian, F.
- Mueller, J.J.
- FES
- MG
- RUA
- 2fe-2s
- Bovine adrenodoxin
- Electron transport
- Intramolecular electron transfer
- Iron
- Iron-sulfur
- Metal-binding
- Mitochondrion
- Ruthenium(ii) bipyridyl complex
- Transit peptide